In order to establish the standard of clinical application and interpretation of these results, the efficacy of mNGS in the diagnosis of pathogens in clinical infectious diseases still needs more comprehensive studies. 8–10 Whether the detection results reflect the real infection status of patient needs to be combined with the clinical situation for comprehensive evaluation since mNGS only detects nucleic acids (including DNA and RNA) in samples. However, the etiological diagnosis of clinical infection remains challenging. In recent years, with the development and application of high-throughput sequencing technology in pathogen detection, mNGS has shown significant advantages compared to other detection methods. It gets rid of the dependence of traditional detection on pathogen isolation and culture, and significantly improves the sensitivity of pathogen detection. 5 With the development of molecular biology technology, nucleic acid detection has become the mainstream development trend of pathogen detection. 4 Serological detection technology based on antigen or antibody is fast and easy to operate, but prior knowledge is necessary, while the detection sensitivity and specificity are also limited. The results of traditional culture method indicated that only 38% of patients with community-acquired pneumonia can be diagnosed by traditional detection method. The traditional pathogen detection technology is mainly based on microbial culture which depends on the vitality of pathogens and media type, incubation temperature, oxygen levels, etc. With the emergence of new pathogenic microorganisms, increasing number of drug-resistant pathogens and immunosuppressive hosts, the incidence and mortality rate of infectious diseases remain high. Infection is one of the main causes of death in critically ill patients. Keywords: metagenomic next-generation sequencing, sensitivity, antibiotic resistance genes, infection, conventional microbiological tests baumannii was of great significance to the anti-infective therapy. And the result of ARGs frequency and cluster analysis of A. It suggested those strains may be the epidemic strains.Ĭonclusion: In our study, mNGS had a higher sensitivity than CMT and culture method. baumannii strains were classified into three groups, and most were well-clustered. High consistency was observed among these ARGs and the related phenotype (100% for ade genes, 91.6% for sul2 and APH(3”)-Ib). baumannii were both detected in mNGS and culture, ade genes were the most frequently detected ARGs (from 13 cases), followed by sul2 and APH(3”)-Ib (both from 12 cases). Importantly, for cases that mNGS-positive only, 18 (35%) cases result in diagnosis modification, and 7 (23%) cases confirmed the clinical diagnosis. Results: Compared with CMT and culture methods, mNGS showed higher sensitivity in pathogen detection (74.2% vs 57.8% P < 0.001 and 66.3% vs 31.7% P < 0.001, respectively). In addition, the antibiotic resistance genes (ARGs) and evolutionary relationship of common drug-resistant A. The pathogen-detection performance of mNGS was compared with conventional microbiological tests (CMT) and culture method. Methods: From December 2019 to March 2021, 134 specimens including Broncho alveolar lavage fluid (BAFL), blood, sputum, cerebrospinal fluid (CSF), bile, pleural fluid, pus, were continuously collected in The First Hospital of Qinhuangdao, and their retrospective diagnoses were classified into infectious disease (128, 95.5%) and noninfectious disease (6, 4.5%). However, the application of mNGS in the diagnosis of clinical pathogens remains challenging. Hongzhi Lu, 1 Li Ma, 1 Hong Zhang, 1 Li Feng, 1 Ying Yu, 1 Yihan Zhao, 1 Li Li, 1 Yujiao Zhou, 1 Li Song, 2, 3 Wushuang Li, 2, 3 Jiangman Zhao, 2, 3 Lanxiang Liu 4ġDepartment of Infectious Diseases, First Hospital of Qinhuangdao, Qinhuangdao, Hebei, 066000, People’s Republic of China 2Shanghai Biotecan Pharmaceuticals Co., Ltd, Shanghai, 201204, People’s Republic of China 3Shanghai Zhangjiang Institute of Medical Innovation, Shanghai, 201204, People’s Republic of China 4Medical Imaging Center, First Hospital of Qinhuangdao, Qinhuangdao, Hebei, 066000, People’s Republic of ChinaĬorrespondence: Lanxiang Liu, Department of Medical Imaging Center, First Hospital of Qinhuangdao, 258 Wenhua Road, Qinhuangdao, 066000, Hebei, People’s Republic of China, Email īackground: Metagenomic next-generation sequencing (mNGS) has been widely studied, due to its ability of detecting all the microbial genetic information unbiasedly in a sample at one time and not relying on traditional culture.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |